Fig. 1

Hsa_circ_0003288 promotes HCC EMT, migration, and invasion. a qRT-PCR analysis of hsa_circ_0003288 expression levels in HCC cell lines HepG2, Huh-7, SMMC-7721, Bel-7402, and human normal hepatic cell line L02. Relative expression levels of hsa_circ_0003288 normalized against β-actin levels. b Hsa_circ_0003288 expression levels in L02 cells transiently overexpressing hsa_circ_0003288 measured by qRT-PCR. Empty vector was used as the negative control. c L02 cells were transfected with hsa_circ_0003288 for 48 h. E-cadherin and N-cadherin protein levels were measured by western blot. β-actin served as the loading control. d Transwell assays were performed on L02 cells transiently overexpressing hsa_circ_0003288. Scale bar, 100 μm. *P < 0.05; **P < 0.01; ***P < 0.001