Fig. 6

Knockdown of hsa_circ_0003288 inhibits PD-L1 expression and inhibits EMT, migration, and invasion of HCC cells. a hsa_circ_0003288 expression levels in HepG2 and Huh7 cells after siRNA transfection. Relative hsa_circ_0003288 expression levels normalized to β-actin levels. b, c PD-L1 mRNA and protein expression levels in HepG2 and Huh7 cells after siRNA transfection. Relative PD-L1 expression levels normalized to β-actin levels. d E-cadherin and N-cadherin protein expression levels in HepG2 and Huh7 cells after siRNA transfection. e Transwell migration and invasion assays of HepG2 and Huh7 cells transfected with siRNA or si-NC. Scale bar, 100 μm. f qRT-PCR analysis of PD-L1 mRNA expression levels after PD-L1 overexpression in si-hsa_circ_0003288 HepG2 and Huh7 cells. g Western blot assay of PD-L1, E-cadherin and N-cadherin, p-AKT and AKT protein levels in HepG2 and Huh7 cells after PD-L1 overexpression in si-hsa_circ_0003288 HepG2 and Huh7 cells. h and i Transwell migration and invasion assays of si-hsa_circ_0003288 HepG2 and Huh7 cells after PD-L1 overexpression. Scale bar, 100 μm. **P < 0.01; ***P < 0.001