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Fig. 2 | Cancer Cell International

Fig. 2

From: circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway

Fig. 2

The role of circ_NRIP1 in ESCC cells in vitro. a RT-qPCR detect circ_NRIP1 expression level in KYSE30 and KYSE450 cells administrated with siRNA target circ_NRIP1 or negative control sequences (simplified as si-circ_NRIP1 or si-NC). b Number of colonies was measured by colony formation assay after transfection. c, d MTS assay determined optical density (OD) of transfected cells at 490 nm after transfection for 0–3 days. e Annexin V-fluorescein isothiocyanate (FITC) and propidium iodide (PI) double staining method and flow cytometry (FCM) analyzed apoptotic rate of transfected cells after transfection for 1 day. f, g Transwell assays evaluated numbers of migrated cells and invaded cells after transfection for 1 day. h, i Western blotting examined protein expression of snail and E-cadherin in transfected cells after transfection for 1 day. *P < 0.05

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