Skip to main content
Fig. 3 | Cancer Cell International

Fig. 3

From: circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway

Fig. 3

The expression of miRNA-595-5p (also known as miR-595) in ESCC tissues and cells. a The circinteractome predicted a complementary site between the wild type of circ_NRIP1 (simplified as circ_NRIP1-wt) and miR-595. Circ_NRIP1-wt was mutated of this potential binding sequence, named as circ_NRIP1-mut. b, c Dual-luciferase reporter assay confirmed luciferase activity of KYSE30 and KYSE450 cells co-transfected with circ_NRIP1-wt/mut and mimics of miR-595 or negative control miRNA (simplified as miR-595 or miR-NC). d RT-qPCR detected miR-595 expression level in KYSE30 and KYSE450 cells introduced with si-circ_NRIP1 or si-NC. e RT-qPCR detected miR-595 expression level in HET-1A, KYSE30 and KYSE450 cells. f RT-qPCR detected miR-595 expression level in Tumor (n = 42) and Normal (n = 42) from ESCC patients. g Pearson’s correlation coefficient assay analyzed the correlation between circ_NRIP1 and miR-595 in ESCC tumor tissues (n = 42). *P < 0.05

Back to article page