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Fig. 4 | Cancer Cell International

Fig. 4

From: circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway

Fig. 4

The reciprocal role of circ_NRIP1 and miR-595 in ESCC cells in vitro. a RT-qPCR detect miR-595 expression level in KYSE30 and KYSE450 cells administrated with si-NC alone, si-circ_NRIP1 alone or combined with anti-RNAs against miR-595 or negative control miRNA (named as anti-miR-595 or anti-miR-NC). b Number of colonies was measured by colony formation assay after transfection. c, d MTS assay determined OD value of transfected cells after transfection for 0–3 days. e Annexin V-FITC and PI double staining method and FCM analyzed apoptotic rate of transfected cells after transfection for 1 day. f, g Transwell assays evaluated numbers of migrated cells and invaded cells after transfection for 1 day. h, i Western blotting examined protein expression of snail and E-cadherin in transfected cells after transfection for 1 day. *P < 0.05

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