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Fig. 6 | Cancer Cell International

Fig. 6

From: circ_NRIP1 is oncogenic in malignant development of esophageal squamous cell carcinoma (ESCC) via miR-595/SEMA4D axis and PI3K/AKT pathway

Fig. 6

The reciprocal role of miR-595 and SEMA4D in ESCC cells in vitro. a, b RT-qPCR and western blotting detect SEMA4D expression levels in KYSE30 and KYSE450 cells administrated with miR-NC alone, miR-595 alone or combined with pcDNA3.1(-) vectors carrying SEMA4D or not (named as SEMA4D or pcDNA) c Number of colonies was measured by colony formation assay after transfection. d, e MTS assay determined OD value of transfected cells after transfection for 0–3 days. f Annexin V-FITC and PI double staining method and FCM analyzed apoptotic rate of transfected cells after transfection for 1 day. g, h Transwell assays evaluated numbers of migrated cells and invaded cells after transfection for 1 day. i, j Western blotting examined protein expression levels of snail and E-cadherin in transfected cells after transfection for 1 day. *P < 0.05

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