Fig. 4

Overexpression of TRIAP1 rescued the inhibitory effect of miR-539 for 143B and MG63 cells. a The expression of TRIAP1 was lower than that in mimic-NC group and mimic + TRIAP1 group. b, c The results of the CCK-8 assay show that the optical density values were lower in the miR-539 mimics group when compared with the miR-539 mimic-NC group and the miR-539 + TRIAP1 overexpression group at 72 h posttransfection in 143b and MG63 cells. d–g The results of the transwell assay show that the invasion ability of 143b and MG63 cells in the miR-539 group was much lower than in the miR-539 mimic-NC group and the miR-539 + TRIAP1 overexpression group. h–k The results of flow cytometry analysis show that the rates of apoptosis were higher in the miR-539 mimics group than in the miR-539 mimic-NC group and the miR-539 + TRIAP1 overexpression group. All of the data show that there was no obvious difference between the miR-539 mimic-NC group and the miR-539 + TRIAP1 overexpression group