Fig. 5

GLCE is low expression in EC and participates in the MONC-miR-636 regulatory axis. a qRT-PCR showed the expression of GLCE in normal endometrial tissue (n = 30) and human endometrial carcinoma (n = 60). b Pearson’s test analyzes the correlation between GLCE and miR-636. c Western blot showed the expression in GLCE normal endometrial tissue (n = 30) and human endometrial carcinoma (n = 60). d GLCE (GLCE-WT) predicted miR-636 binding site and the designed mutant sequence (GLCE-Mut). Cells were transfected with GLCE-WT or GLCE-Mut and the indicated miR-636, and then subjected to dual luciferase reporter gene analysis. e Western blot was used to observe the effects of MONC and miR-636 on GLCE expression in ECSCs, and Ishikawa and HEC-1A cell lines. The data are expressed as mean ± SEM (n = 3, each group). * P < 0.05, ** P < 0.01, and *** P < 0.001