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Fig. 6 | Cancer Cell International

Fig. 6

From: MicroRNA-665 facilitates cell proliferation and represses apoptosis through modulating Wnt5a/β-Catenin and Caspase-3 signaling pathways by targeting TRIM8 in LUSC

Fig. 6

TRIM8 overexpression suppresses the proliferation of human LUSC cells. a TRIM8 overexpression increases TRIM8 mRNA levels in NCI-H226/SK-MES-1 cells. b CCK8 assay showed that TRIM8 overexpression repressed NCI-H226/SK-MES-1 cell proliferation. c Cell cycle was detected in NCI-H226/SK-MES-1 cells at 24 h after transfection. d The percentage of sub-G1 cells population changed after transfection with TRIM8 overexpression vector. e Apoptosis was measured in NCI-H226/SK-MES-1 cells at 48 h after transfection. f Wnt5a, β-Catenin, c-Myc, Cyclin D1 and active Caspase-3 protein levels were detected after TRIM8 overexpression vector transfection. g TRIM8 overexpression affected TOPFLASH TCF-reporter construct activity in NCI-H226/SK-MES-1 cells. h CCK8 assay revealed cell proliferation after co-transfection with TRIM8 and Wnt5a overexpression vectors in NCI-H226/SK-MES-1 cells. n = 3, *p < 0.01, **p < 0.001, as compared with Empty Vector-transfected cells; #p < 0.01, as compared with TRIM8 overexpression Vector-transfected cells

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