Fig. 4

lncRNA-APOC1P1-3 binds to miRNA-188-3p, and miRNA-188-3p reverses the anti-apoptotic effect of LincRNA-APOC1P1-3. a Venn diagram of target microRNAs predicted by multiple databases. b RNA-chip predicted binding sites of APOC1P1-3 with miRNA-188-3p. c The overexpression of transfected miRNA 188-3p mimics was detected by qPCR, which was significantly higher than that in the control group (n = 3, **P < 0.01). d Combination of APOC1P1-3 with miRNA-188-3P. When the pmirGLO-APOC1P1-3′UTR plasmid and miRNA-188-3P mimics were co-transfected, the luminescence signal significantly decreased. When the pmirGLO-APOC1P1-Mut plasmid and miRNA-188-3P mimics were co-transfected, the luminescence signal was restored. e Compared with the control group, early apoptosis of MDA-MB-231a cells transfected with miRNA-188-3p mimics increased significantly (FACS). f Overexpression of miRNA-188-3p followed by overexpression of lncRNA- APOC1P1-3 reversed its pro-apoptotic effect(FACS). g, h There’s no difference in the expression level of lncRNA- APOC1P1-3 after overexpressing miRNA-188-3p (qRT-PCR). After overexpressing lncRNA- APOC1P1-3, the expression of miRNA-188-3p was also unchanged