Fig. 2

Alkaline phosphatase (ALPL) has no effect on the proliferation of LUAD cells. Western blot analysis of A549 (a) and HCC827 (b) cells stably transfected with an ALPL overexpression plasmid or a control plasmid. GAPDH was used as an internal control. Soft-agar assay to determine the effect of ALPL overexpression on anchorage-independent growth of A549 (c, d) and HCC827 (e, f) cells. Representative images of colonies were captured by microscopy after 3 weeks of incubation (c, e), Scale bars: 200 µm. The number of colonies with > 32 cells were counted in A549 (Vector) and A549 (ALPL) cells (d), and in HCC827 (Vector) and HCC827 (ALPL) cells (f). The results represent the number of colonies per 10,000 cells. Data were presented as the mean ± SD and analyzed by Student′s t-test, n = 3. ns, no statistical significance relative to the vector control cells (P > 0.05)