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Fig. 6 | Cancer Cell International

Fig. 6

From: Alkaline phosphatase downregulation promotes lung adenocarcinoma metastasis via the c-Myc/RhoA axis

Fig. 6

Alkaline phosphatase (ALPL) promotes the degradation of c-Myc by activating ERK. a qPCR analysis of the levels of c-Myc mRNA in A549 and HCC827 cells overexpressing ALPL. ns, not significant. b The rate of c-Myc protein degradation in A549 (Vector) and A549 (ALPL) cells. GAPDH was used as an internal control. c Western blot analysis of the levels of c-Myc and p-c-Myc (Thr58/Ser62) in A549 (Vector) and A549 (ALPL) cells after treatment with MG132. d Western blot analysis of the indicated cells for levels of the ubiquitin-related molecules ERK1/2, GSK-3β, p-ERK1/2, and p-GSK-3α/β. GAPDH was used as an internal control. e Western blot analysis of the levels of p-ERK1/2, ERK1/2, c-Myc, and p-c-Myc (Thr58/Ser62) in A549 (ALPL) cells after treatment with PD98059 (100 μM)

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