Fig. 5
From: TriBAFF-CAR-T cells eliminate B-cell malignancies with BAFFR-expression and CD19 antigen loss
TriBAFF-CAR-T cells have the strong cytotoxicity to the B cell line with CD19 knockout in vitro. a Raji with CD19KO was CD19-negative. b Raji with CD19KO was still expressed in BAFFR. c Lysis of Raji with CD19KO was detected after coculturing with TriBAFF-CAR-T or CD19-CAR-T cells at different effector: target ratios. d To stimulate the relapse BLL, Raji and Raji with CD19KO were mixed at a ratio of 4:1. e The relative proliferation of mixed WT and CD19KO Raji was detected after coculturing with TriBAFF-CAR-T or CD19-CAR-T cells for 48 h. f Flow cytometry indicated that mixed WT and CD19KO Raji were alive and BAFFR-positive after coculturing with CD19-CAR-T cells. g A flowchart for the research methodology