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Fig. 4 | Cancer Cell International

Fig. 4

From: Cyproheptadine, an epigenetic modifier, exhibits anti-tumor activity by reversing the epigenetic silencing of IRF6 in urothelial carcinoma

Fig. 4

IRF6 inhibited cell proliferation and induced apoptosis in UC cells. a RT-PCR showing IRF6 expression was depleted by lentiviral shRNA knockdown at two different regions in TSGH8301 and BFTC905 UC cells. Colony formation assay shows that depletion of IRF6 increased tumor growth in (b) TSGH8301 and (c) BFTC905 cells. Right panel indicates the quantitative analysis of the colony forming assay in each cells. d Quantitative RT-PCR confirmed overexpression of IRF6 in UMUC3 (Left panel) and J82 (Right panel) cells transiently transfected with IRF6 overexpressing plasmid. Overexpression of IRF6 inhibited tumor growth by colony formation assay in (e) UMUC3 and (f) J82. Right panel indicates the quantitative analysis of the colony forming assay in each cells. Data are expressed as mean ± SD in triplicates (*p < 0.05, ***p < 0.001). g The percentage of apoptotic cells in UMUC3 and cells overexpressed with IRF6, were analyzed by Annexin V/7-AAD assay using flow cytometry. The upper right quadrant (Q2) represents non-viable late apoptotic cells. Right panel indicates quantitative analysis of apoptotic cells in two independent experiments (mean ± SD)

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Cancer Cell International

ISSN: 1475-2867

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