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Fig. 3 | Cancer Cell International

Fig. 3

From: ESR1 as a recurrence-related gene in intrahepatic cholangiocarcinoma: a weighted gene coexpression network analysis

Fig. 3

The effect of ESR1 on iCCA cell proliferation, migration and invasion. a The IHC results for ESR1 in iCCA tissues and normal liver tissues based on the Human Protein Atlas database and samples from the validation cohort. ESR1 was significantly overexpressed in normal liver tissue vs. iCCA tissue. b Survival analysis of the association between ESR1 expression level and DFS based on the TCGA database. Low ESR1 expression was associated with a poor prognosis in iCCA patients. c Comparison of ESR1 expression levels in iCCA cell lines (QBC939, RBE, HCCC-9810, and FRH0201) and a normal biliary epithelial cell line (HiBEpiC). The RBE cell line displayed the highest expression levels, and the HCCC-9810 cell lines displayed the lowest expression levels of the four iCCA cell lines. The HCCC-9810 cell line was chosen to conduct a gain-of-function experiment, and the RBE cell line was chosen to conduct a loss-of-function experiment. d The ESR1 expression level of the RBE cell line in the Si-ESR1 group and NC-ESR1 group. The ESR1 expression level of HCCC-9810 cells in the Ov-ESR1 group and the NC-ESR1 group. e CCK-8 assay of RBE and HCCC-9810 cells. f Transwell assay of HCCC-9810 and RBE cells. g Colony formation assay of RBE and HCCC-9810 cells. h The expression of essential molecules in the JAK/STAT3 signaling pathway after upregulating the expression of ESR1 in the HCCC-9810 cell line

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