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Fig. 3 | Cancer Cell International

Fig. 3

From: LINC01158 works as an oncogene in glioma via sponging miR-6734-3p to boost CENPK expression

Fig. 3

LINC01158 functioned as the sponge of miR-6734-3p to enhance CENPK expression. a Subcellular fractionation followed by qRT-PCR examined LINC01158 distribution in the nucleus or cytoplasm of glioma cells. b FISH assay determined the location of LINC01158 in glioma cells. Scale bar = 10 μm. c DIANA and miRDB predicted miR-6734-3p and miR-4475 as the potential partners of both LINC01158 and CENPK. d The miR-6734-3p binding sites within LINC01158 and CENPK were separately predicted by DIANA-lncBase and miRDB. e Luciferase reporter assay investigated the luciferase activity in glioma cells with miR-6734-3p mimics (or NC-mimics) and LINC01158-WT/MUT or CENPK-WT/MUT reporters. f RIP assay suggested LINC01158, miR-6734-3p and CENPK could associate with Ago2 protein. g qRT-PCR illustrated that downregulating miR-6734-3p reversed the suppressed trend of CENPK mRNA altered by LINC01158 depletion. **and *** note P < 0.01 and P < 0.001, respectively

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