Fig. 6

c-Jun plays an important role in the up-regulation of Talin2 mRNA and miR-190 level. a miR-190 is located in the intron region of the Talin2 gene. b Relative Talin2 mRNA expression was detected in UROtsa and UMUC3 cells. *significant difference (*P < 0.05, **P < 0.01, ***P < 0.001). c The promoter activity of Talin2 was evaluated and compared between UROtsa and UMUC3 cells. *significant difference (*P < 0.05, **P < 0.01, ***P < 0.001). d Potential transcriptional factor-binding sites in the Talin2 promoter region (– 887 to +328) analyzed by using the ALGGEN engine online. e The indicated stable transfectants were subjected to Western Blot to determine p-C-Jun Ser73, C-Jun, C-Fos, Ets-1, Elk-1, and JunB levels. GAPDH was used as a protein loading control. f Relative AP-1 activity was detected in UROtsa and UMUC3 cells. *significant difference (*P < 0.05, **P < 0.01, ***P < 0.001). g c-Jun dominant-negative mutant TAM67 and vector control plasmids were transfected into UMUC3 cells, and c-Jun dominant-negative mutant was identified by western blot. h–j Relative Talin2 promoter activity (h), Talin2 mRNA (i) and miR-190 level (j) detected in TAM67 was compared to that in their vector control cells. *significant difference (*P < 0.05, **P < 0.01, ***P < 0.001). k The proposed schematic for the mechanism underlying miR-190 contributes to Human Bladder Cell Transformation