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Fig. 2 | Cancer Cell International

Fig. 2

From: Dendritic cells matured with recombinant human sperm associated antigen 9 (rhSPAG9) induce CD4+, CD8+ T cells and activate NK cells: a potential candidate molecule for immunotherapy in cervical cancer

Fig. 2

Optimization of rhSPAG9 antigen concentration for priming DCs (a) Percentage of mature DCs yield at different concentrations of rhSPAG9 priming. Comparative analysis of unprimed DCs and different concentration of rhSPAG9 primed DCs (b) Phenotypic characterization (c) Proliferation of allogenic CD4+ and CD8+ Tcells (PBMCs from N1–N5 healthy controls) (d) Overall proliferation of autologous PBMCsin response to SPDCs and unprimed DCs (P4–P5) (e) Proliferation of autologous CD4+and CD8+ Tcellsupon stimulation with SPDCs and unprimed DCs (f) Secretion level of IL12p40 in wash out (WO) and co-cultures supernatants of SPDCs and unprimed DCs (g) Secretion level of IFNγ by autologous PBMCsco-cultured withSPDCs and unprimed DCs (P4–P5). For Figs. 2(a–c), data represents summary of five different experiments (P1–P5)

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Cancer Cell International

ISSN: 1475-2867

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