Fig. 4

Functional characterization of mature DCs in allogenic and autologous co-cultures (a) Overall proliferation of allogenic PBMCs induced by SPDCs and TLDCs (P = 0.999) (b) Proliferation of allogenic CD4⁺ and CD8⁺ T cells in co-culture with DCs (N1-N3) (c) Representative histogram of overall proliferation of autologous PBMCs induced by SPDCs and TLDCs (d) Proliferation of autologous CD4⁺, CD8⁺ T cells and NK cells in response to SPDCs and TLDCs(CD4⁺; P = 0.400, CD8⁺; P = 0.700, CD56⁺; P = 0.700). e Secretion of IFNγ by allogenicPBMCs co-cultured supernatants of SPDCs and TLDCs (N1-N3: P = 0.700) (f) Secretion of IFNγ by autologous PBMCs co-cultured supernatants of SPDCs and TLDCs (P7-P9; P = 0.400) (g) The level of IL12p40in autologous wash out andco-cultured supernatants of SPDCs and TLDCs(WO—Wash out; P = 0.685 and at day7; P = 0.485) (h) The level of IL12p70 (WO—Wash out; P = 0.999 and at day7; P = 0.914) in wash out andautologous co-cultured supernatants of SPDCs and TLDCs. All individual experiments were done in duplicates and data shown from all autologous experiments is from three different patients for the SPDCsand TLDCs experiments (P7–P9)