Fig. 9

CircHECTD1 mediated the sensitivity of GC cells to DB treatment by regulating PBX3 in GC. a–g AGS and HGC-27 cells under DB treatment were transfected with si-NC, si-circHECTD1, si-circHECTD1 + pcDNA, or si-circHECTD1 + PBX3, respectively. a, b The mRNA and protein levels of PBX3 in transfected GC cells were detected by qRT-PCR and western blot assays. c, d IC50 values (µM) of transfected cells were determined by MTT assays. e, f Flow cytometry assay was conducted to estimate cell cycle distribution and cell apoptosis. g The protein levels of Bcl-2 and Bax in cells upon transfection were detected by western blot. All the data from three different experiments were shown as means ± SD. *P < 0.05