Table 2 Sequences of siRNA, shRNA and oligonucleotides used in this research
From: Circular RNA circHECTD1 prevents Diosbulbin-B-sensitivity via miR-137/PBX3 axis in gastric cancer
Gene | siRNA Sequence (5’-3’) |
|---|---|
si-circHECTD1 | CGGTTGTACGCAAGGTTGATC |
si-NC | |
 Sense | GAUUGUUCUGAGAGACGAAGA |
 Antisense | UUCGUCUCUCAGAACAAUCUG |
si-PBX3 | |
 Sense | UUCUUCGAACGUGUCACGUTT |
 Antisense | ACGUGACACGUUCGGAGAATT |
anti-miR-137 | CUACGCGUAUUCUUAAGCAAUAA |
anti-miR-NC | GGUUCGUACGUACACUGUUCA |
miR-137 | UUAUUGCUUAAGAAUACGCGUAG |
miR-NC | GGUUCGUACGUACACUGUUCA |
sh-circHECTD1 | |
 Sense | GATCCGCACGGTTGTACGCAAGGTTGTTCAAGAGACAACCTTGCGTACAACCGTGTTTTTTGGAAG |
 Antisense | AATTCTTCCAAAAAACACGGTTGTACGCAAGGTTGTCTCTTGAACAACCTTGCGTACAACCGTGCG |
sh-NC | |
 Sense | CACCGTTCTCCGAACGTGTCACGTCAAGAGATTACGTGACACGTTCGGAGAATTTTTTG |
 Antisense | GATCCAAAAAATTCTCCGAACGTGTCACGTAATCTCTTGACGTGACACGTTCGGAGAAC |