Fig. 3

Knocking down circRNA_100290 inhibits GC cell viability and colony formation and induces G0/G1 cycle arrest. A Expression of circRNA_100290 was detected in four GC cell lines, AGS, BGC823, SGC7901, HGC27, and GES-1 human immortalized normal gastric epithelial cells by qRT-PCR assay. B Expression of miR-29b-3p was detected in the AGS, BGC823, SGC7901, HGC27 and GES-1 cell lines by qRT-PCR assay. C Detection of miR-29b-3p and ITGA11 expression in the AGS cell line by qRT-PCR assay after circRNA_100290 siRNA transfection was conducted. D Detection of miR-29b-3p and ITGA11 expression in the HGC-27 cell line by qRT-PCR assay after circRNA_100290 siRNA transfection was conducted. E, F CCK-8 assays were used to evaluate the effects of circRNA_100290 on the proliferation of AGS and HGC27 cells. Knocking down circRNA_100290 expression significantly inhibited GC cell viability. ***P < 0.001. G, I Colony formation assays were performed to evaluate the effects of circRNA_100290 on the colony-formation abilities of AGS and HGC27 cells. H, J The statistical analysis of the graphs showed that decreasing circRNA_100290 expression suppressed the AGS and HGC27 cell colony formation ability (*P < 0.05). K, M Flow cytometry was used to analyse the effects of circRNA_100290 on AGS and HGC27 cell cycle progression. L, N The statistical analysis of the graphs demonstrated that decreasing circRNA_100290 expression increased the percentage of AGS and HGC27 cells in the G0/G1 phase compared with the NC control groups (*P < 0.05)