Fig. 6

Silencing the expression of LPCAT1 inhibits the proliferation of endometrial cancer cells. Volcano plot, protein–protein interaction and enrichment analyses of GO and KEGG for DEGs between the high-expression group and low-expression group of LPCAT1. a Representative image of LPCAT1 expression in endometrial cancer tissues and normal endometrial samples was evaluated by immunohistochemistry. b Integrated optical density was analysed. ****P < 0.0001. c Western blotting was performed to evaluate the protein expression of LPCAT1 at the translation level after transfection with a siRNA targeting LPCAT1 or a scrambled siRNA as a negative control (si-NC). d The relative proliferation ability of the transfected endometrial cancer cells was detected at a fixed time for 5 days by the MTT assay. The growth curves were analysed using 2-way ANOVA. e Volcano plot of the DEGs generated by comparison of the high expression group and low expression group depending on the median expression of LPCAT1. Differentially expressed genes were determined by the Wilcoxon rank sum test with q = 0.05 and log2FC transformation as the significance threshold. f GO and KEGG enrichment analyses of 378 DEGs. Terms with p and q < 0.05 were considered to be significantly enriched. g Interaction network constructed with nodes with interaction confidence values > 0.65