Fig. 3
From: The mutual regulatory loop between TPTEP1 and miR-1303 in leukemogenesis of acute myeloid leukemia
JUN positively regulated miR-1303 in AML cells at transcriptional level. a JASPAR predicted that there existed four possible binding sites of c-JUN and microRNA(miR)-1303 promoter. b The expression level of JUN in HL60, KG-1 and normal control HS-5 cells was examined with the help of qRT-PCR. c, d The expression of JUN and miR-1303 in HL60 and KG-1 cells with or without JUN silence was determined through qRT-PCR. e The effect of c-JUN on miR-1303 transcription was assessed by chromatin immunoprecipitation (ChIP) and luciferase reporter assays. f–g Luciferase reporter assay was implemented to verify the precise binding site of c-JUN to miR-1303 promoter. Data were presented as mean ± SD (error bar). **P < 0.01