Fig. 1

The lymphocyte subsets and immunophenotypic characteristics. Circulating lymphocytes in patients newly diagnosed with DLBCL and healthy controls (HCs) were analyzed using flow cytometry. A, B The percentages and absolute numbers of T cells, B cells and NK cells in different groups are shown in bar graphs (mean ± SD). C The expression of CD28 on CD4⁺ and CD8⁺ T cells, the expression of HLA-DR on CD3⁺ and CD8⁺ T cells, and the expression of CD45RO and CD45RA on CD4⁺ T cells in different groups are shown in bar graphs (mean ± SD). D The percentages of Tregs (CD4⁺ CD25⁺ CD127^low) in lymphocytes are shown in bar graphs (mean ± SD). (E) The percentages of CD69 in NK cells are shown in bar graphs (mean ± SD). *p < 0.05, **p < 0.01, ***p < 0.001