Fig. 3

Blocking HO-1 sensitizes resistant MCL cells to BTZ by enhancing cytotoxic ROS levels and reducing cytoprotective autophagy formation. REC-1 (a) and BACH2^KD Jeko cells (b) were treated with BTZ (20 nM) with or without 1 h-pretreatment of SnPP (5 μM) for 24 h. Cells treated with DMSO or SnPP were used as negative controls. Cell viability was measured using MTT assays. Data are normalized to DMSO-treated cells and shown as the mean ± SD from three independent experiments. **p < 0.01; ***p < 0.001 (vs DMSO-treated group or single BTZ-treated group). c ROS assessment in resistant cells treated with BTZ (20 nM) in the presence or absence of SnPP (5 μM) for 24 h. Data are normalized to single BTZ-treated cells and shown as the mean ± SD from two independent experiments. **p < 0.01 (vs single BTZ-treated group). LC3II levels were measured using immunoblotting in REC-1 (d) and BACH2^KD Jeko cells (e) treated with BTZ (20 nM) with or without SnPP pretreatment (5 μM) for 24 h, respectively. Actin was used as a loading control, and the normalized values (LC3II/Actin) are indicated