Fig. 8

Knockdown of COL3A1 inhibiting migration, invasion, and EMT process. A Relative expression of COL3A1 in glioma (LGG and GBM) and normal brain tissue. After COL3A1 siRNA transfected, SHG44 and A172 (B) cells showed reduced COL3A1 levels measured by qRT-PCR. C Wound healing assays indicated that the migratory ability of glioma cells was reduced after transfection. D Transwell assays revealed that the invasiveness ability of glioma cells was inhibited after transfection. E-F The protein levels of Vimentin and N-cadherin were detected by Western blotting analysis in SHG44 and A172 cells after COL3A1 knockdown with siRNA. Data represented as mean value ± SD. * p < 0.05, ** p < 0.01