Comparison of characteristics of differentiated NK cells. A and B assessment of CD56/CD16
and CD3 markers expression among all 4 groups (IL-7+IGF-1, IL-21+IGF-1, IL7+IL-21+IGF-1 and IL-7+IL-21+SIS3+IGF-1) showed a significant expression difference in the groups IL-7+IL-21+ IGF-1 (P < 0.01) and IL-7+IL-21+ IGF-1+SIS3 (P < 0.01). C, these 2 groups also demonstrated significant difference in the expression of NKG2D compared to the other groups. D, the expansion potential analysis showed that IL-7+IL-21+ IGF-1+SIS3 group had the highest level of Fold expansion among all groups (P < 0.05). It should be noted that all expressions were assessed in the viable cells (>90%±3.00). P<0.01 was considered
significant (*P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 and ****P ≤ 0.0001). Data is demonstrated as mean ± SD, n = 3.