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Table 1 Culture media in different phases of NK generation

From: Overcoming the UCB HSCs –Derived NK cells Dysfunction through Harnessing RAS/MAPK, IGF-1R and TGF-β Signaling Pathways

Phase Cell culture medium Concentration (ng/ml) Exposure time (day)
Expansion SCF 50 7
TPO 50
FLt3-L 50
GM-CSF 50
rhIL-3 25
rhIL-6 25
Differentiation SCF 25 10
TPO 25
rhIGF-1 50
rhIL-7 25
rhIL-21 25
SIS3 (Smad3 inhibitor) 0.125
Activation SCF 2.5 4
TPO 2.5
rhIGF-1 5
rhIL-7 10
rhIL-15 50
rhIL-21 10
SIS3 (Smad3 inhibitor) 0.125
rhHsp70 2000
  1. SFT (SCF/ FLt3-L/TPO), SFT36G (SCF/ FLt3-L/TPO/IL-3/IL-6/GM-CSF)
  2. I7 (IGF-1/IL-7), I21 (IGF-1/IL-21), I721 (IGF-1/IL-7/IL-21), IS721 (IGF-1/SIS3/ IL-7/IL-21)