Table 1 Culture media in different phases of NK generation
Phase | Cell culture medium | Concentration (ng/ml) | Exposure time (day) |
|---|---|---|---|
Expansion | SCF | 50 | 7 |
TPO | 50 | ||
FLt3-L | 50 | ||
GM-CSF | 50 | ||
rhIL-3 | 25 | ||
rhIL-6 | 25 | ||
Differentiation | SCF | 25 | 10 |
TPO | 25 | ||
rhIGF-1 | 50 | ||
rhIL-7 | 25 | ||
rhIL-21 | 25 | ||
SIS3 (Smad3 inhibitor) | 0.125 | ||
Activation | SCF | 2.5 | 4 |
TPO | 2.5 | ||
rhIGF-1 | 5 | ||
rhIL-7 | 10 | ||
rhIL-15 | 50 | ||
rhIL-21 | 10 | ||
SIS3 (Smad3 inhibitor) | 0.125 | ||
rhHsp70 | 2000 |