Fig. 3

Circ_0006168 regulated JMJD1C expression by acting as a sponge of miR-194-5p. A Predicted binding sites between miR-194-5p and circ_0006168 were shown. B The expression of miR-194-5p was analyzed by qRT-PCR in Eca109/Taxol and KYSE150/Taxol transfected with si-control and si-circ_0006168. C The level of miR-194-5p was examined by qRT-PCR in Eca109/Taxol and KYSE150/Taxol cells transfected with miR-NC or miR-194-5p. D Dual-luciferase reporter assay was performed to determine the luciferase activity in Eca109/Taxol and KYSE150/Taxol cells co-transfected with miR-NC or miR-194-5p and circ_0006168-wt or circ_0006168-mut. E Circ_0006168 and miR-194-5p enrichment in Eca109/Taxol and KYSE150/Taxol were analyzed by RIP assay. F Circ_0006168 enrichment in Eca109/Taxol and KYSE150/Taxol transfected with NC-input, bio-NC, bio-miR-194-5p, or miR-194-5p-input was detected using RNA pull-down assay. G The level of miR-194-5p was detected by qRT-PCR in ESCC tissues and adjacent normal tissues. H The expression of miR-194-5p was examined by qRT-PCR in HET-1A cells, parental cells (Eca109 and KYSE150), and Taxol-resistant cells (Eca109/Taxol and KYSE150/Taxol). I The putative binding sites between miR-194-5p and JMJD1C were predicted by TargetScan. J The luciferase activity was determined in Eca109/Taxol and KYSE150/Taxol co-transfected with miR-NC or miR-194-5p and JMJD1C-wt or JMJD1C-mut. K JMJD1C and miR-194-5p enrichment in Eca109/Taxol and KYSE150/Taxol were measured by RIP assay. L Circ_0006168 enrichment was determined by RNA pull-down assay in Eca109/Taxol and KYSE150/Taxol cells transfected with NC-input, bio-NC, bio-miR-194-5p, or miR-194-5p-input. M The expression of miR-194-5p was examined by qRT-PCR Eca109/Taxol and KYSE150/Taxol cells transfected with inhibitor-NC or inhibitor-miR-194-5p. N The protein expression of JMJD1C was tested by western blot analysis in Eca109/Taxol and KYSE150/Taxol transfected with miR-NC, miR-194-5p, inhibitor-NC, or inhibitor-miR-194-5p. O Western blot assay was carried out to analyze the protein expression of JMJD1C in Eca109/Taxol and KYSE150/Taxol transfected with si-control, si-circ_0006168, si-circ_0006168 + inhibitor-NC, si-circ_0006168 + inhibitor-miR-194-5p. *P < 0.05, **P < 0.001, ***P < 0.0001