Fig. 4
JMJD1C silence improved Taxol sensitivity in Taxol-resistant cells. A The mRNA expression of JMJD1C was analyzed by qRT-PCR in ESCC tissues and adjacent normal tissues, and western blot assay was performed to measure the protein expression of JMJD1C in HET-1A cells, parental cells (Eca109 and KYSE150), and Taxol-resistant cells (Eca109/Taxol and KYSE150/Taxol). B–I Eca109/Taxol and KYSE150/Taxol cells were transfected with si-control or si-JMJD1C. B The protein expression of JMJD1C was detected by western blot assay. C, D CCK-8 assay was employed to detect cell viability and IC50 value of Taxol. E Cell viability was measured using CCK-8 assay. F Colony formation assay was utilized to assess cell colony formation ability. G, H The migration and invasion capacities were evaluated using transwell assay (× 100). I Flow cytometry analysis was utilized to determine cell apoptosis. *P < 0.05, **P < 0.001, ***P < 0.0001