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Fig. 10 | Cancer Cell International

Fig. 10

From: circ-PTK2 (hsa_circ_0008305) regulates the pathogenic processes of ovarian cancer via miR-639 and FOXC1 regulatory cascade

Fig. 10

Validation of the interaction between miR-639 and FOXC1. A Reverse transcriptase-quantitative PCR validation of the level of FOXC1 mRNA when miR-639 was inhibited or overexpressed. B Binding sequences between FOXC1 mRNA and miR-639 were predicted, and wild-type and mutated FOXC1 genes were synthesized and cloned into luciferase vector to generate a reporter. C Luciferase activity was determined to validate the interaction between FOXC1 mRNA and miR-639. D Transwell assay with SK-OV-3 cells showing enhanced cell migration and invasion due to overexpression of circ-PTK2 while inhibition of FOXC1 mRNA expression suppressed those processes. E Transwell assay with OVCAR-3 cells showing enhanced cell migration and invasion due to overexpression of circ-PTK2 while inhibition of FOXC1 mRNA expression suppressed those processes. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001

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Cancer Cell International

ISSN: 1475-2867

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