Fig. 3

LncRNA WDFY3-AS2 induced the traits of CSCs. A Representative A2780-DDP sphere-forming cells were imaged (100 μm). A2780-DDP cells were treated for 7 days with 20 μM cisplatin. B Western blotting was used to detect the CSC markers protein expression including the SOX2, OCT4 and Nanog in A2780-DDP sphere-forming cells. C Detection of CD44⁺, CD166⁺-positive cells in A2780-DDP sphere-forming cells via flow cytometry. **P < 0.01 vs. control, ***P < 0.001 vs. control