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Fig. 4 | Cancer Cell International

Fig. 4

From: Long non-coding RNA LINC01116 is activated by EGR1 and facilitates lung adenocarcinoma oncogenicity via targeting miR-744-5p/CDCA4 axis

Fig. 4

LINC01116 mediates CDCA4 expression via competitively binding to miR-744-5p. A qRT-PCR analysis was applied for quantifying expression levels of potentially combinable mRNAs of miR-744-5p in cells with miR-744-5p augment. Student’s t test was applied for analysis. B CDCA4 expression in LAD cell lines was determined by means of qRT-PCR. One-way ANOVA was applied for analysis. C Putative and mutant binding sites of miR-744-5p with CDCA4 were depicted. D It was reflected in Dual luciferase reporter assays that the decline in luciferase activity of CDCA4-WT resulted frommiR-744-5p mimics transfection. Student’s t test was applied for analysis. E The expression of LINC01116 was explored after transfection of pcDNA3.1/LINC01116 in A549 and PC9. Student’s t test was applied for analysis. F QRT-PCR based on RIP indicated that LINC01116 competed against CDCA4 for the binding with miR-744-5p. One-way ANOVA was applied for analysis. G, H The effects of miR-744-5p and LINC01116 overexpression on CDCA4 mRNA and protein levels were evaluated by qRT-PCR and western blot. One-way ANOVA was applied for analysis. *P  <  0.05, **P  <  0.01

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