Fig. 5

LINC01116 facilitates LAD oncogenicity via targeting miR-744-5p/CDCA4 axis. A The expression of CDCA4 was markedly up-regulated by pcDNA3.1/CDCA4. Student’s t test was applied for analysis. B, C EdU and colony formation assays were performed in A549 to study the effects of CDCA4 up-regulation on sh-LINC01116 induced cell proliferation. One-way ANOVA was applied for analysis. D Flow cytometry was conducted to evaluate cell apoptosis in a rescue analysis. One-way ANOVA was applied for analysis. E The variation of apoptosis-related proteins was investigated via western blot in a rescue analysis. F, G Transwell assays were performed for investigation into effects of CDCA4 up-regulation on sh-LINC01116 induced cell migration and invasion. One-way ANOVA was applied for analysis. H Xenograft tumor growth curve over 4 week was illustrated after A549 cells inoculation. One-way ANOVA was applied for analysis. I, J Xenograft tumor volume and weight were measured after inoculation of A549 cells transfected with different plasmids respectively. One-way ANOVA was applied for analysis. **P  <  0.01