Fig. 2
From: IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells
Effect of IFI35 on X-ray irradiation and the proliferation and colony formation of colorectal cancer cells. A CCK8 was used to detect the effect of IFI35 on the proliferation of colorectal cancer cells. Overexpression of IFI35 decreases colorectal cancer cell viability, while the inhibition of IFI35 increases their viability. B Flow cytometry detection of the effect of IFI35 on the cell cycle of colorectal cancer cells. The bar graph shows the proportion of cells in each cell cycle phase. Overexpressing IFI35 increases the proportion of cells in G2, while inhibiting IFI35 decreases the proportion of cells in G2. C The effect of IFI35 on the colony formation rate of colorectal cancer cells was verified by the colony formation test. The bar graph shows the colony formation rates. Overexpression IFI35 decreases the colony formation, while inhibiting IFI35 increase colony formation. *p < 0.05, **p < 0.01, ***p < 0.001 vs. Ad-NC or shNC, as appropriate. Ad-NC: adenovirus, negative control; Ad-IRF1, adenovirus for IRF1 overexpression; sh: short hairpin RNA for the downregulation of IRF1 or IFI35. shNC: negative control short hairpin RNA