Fig. 5
From: IFI35 is involved in the regulation of the radiosensitivity of colorectal cancer cells
In vivo experiments supporting the effect of colorectal cancer on X-ray radiosensitivity after IFI35. Four-week-old BALB/c nude mice were divided into five groups: (1) Control group without treatment; (2) Ad-IFI35 group; (3) Ad-NC group; (4) shRNA-IFI35 group; and (5) shRNA-NC group (n = 5/group). A Tumor volume growth curve of each group. Two-dimensional measurements were taken with an electronic caliper every 3 days, and the tumor volume in mm3 was calculated. B Representative subcutaneous tumors of each group. C Liver metastasis models were established by tail vein injection of IFI35-up/down-regulated and control CCL244 cells (grouping was the same as above). Liver metastasis specimens of colorectal cancer and HE staining of liver metastases in each group. The red arrows point at metastatic lesions in the specimen and the HE staining. D Immunohistochemistry was used to detect the expression of the apoptotic protein Bcl-2 in subcutaneous tumors and liver metastases. Ad-NC: adenovirus, negative control; Ad-IRF1, adenovirus for IRF1 overexpression; sh: short hairpin RNA for the downregulation of IRF1 or IFI35. shNC: negative control short hairpin RNA