Fig. 1

Identification and validation of differential circNALCN expression in glioma tissues and cells. A Volcano plot of differentially expressed circRNAs. The blue dots represent downregulated circRNAs, whereas the red dots represent upregulated circRNAs in glioma compared with normal tissue. circNALCN is labeled as hsa_circ_0099761. B Schematic showing the circularization of NALCN exons 2–7 in the formation of circNALCN. C qRT-PCR verified that the expression level of circNALCN in glioma tissues was lower than that in normal brain tissues in the sequenced specimens. D circNALCN expression was detected via qRT-PCR in 76 cases of glioma tissues and 17 cases of normal brain tissues. E circNALCN is expressed in WHO Grade I–II and WHO Grade III–IV glioma. F circNALCN expression was detected via qRT-PCR in various glioma cell lines (U87, U251, and SF126) and human normal glial cells (HEB). Student’s t-test, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001