Fig. 2

Characterization and clinical features of circNALCN. A Nucleic acid electrophoresis confirmed that circNALCN is a circular RNA. circNALCN was amplified by divergent primers in cDNA but not gDNA. B, C The expression of circNALCN and linear NALCN mRNA in both U87 and U251 cell lines was detected by qRT-PCR in the presence or absence of RNase R. D FISH assay showed that circNALCN was predominantly localized in the cytoplasm. Nuclei were stained with DAPI in blue, and cytoplasmic circNALCN were stained in green. (magnification, 400×, scale bar, 20 μm). E Kaplan–Meier survival curves of glioma patients with low and high circNALCN expression levels, using the median circNALCN value as a cutoff. Data are showed as the mean ± SD; ns indicates no significance, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001