Fig. 5

circNALCN acts as an miRNA sponge for miR-493-3p, and the antitumor effect of circNALCN in glioma can be reversed by miR-493-3p. A Schematic illustration of circNALCN-WT and circNALCN-MUT luciferase reporter vectors. B, C After co-transfection with circNALCN-WT or circNALCN-MUT and miR-93-3p or NC mimics, the relative luciferase activity of U87 and U251 cells were detected. D FISH was performed to observe the cellular location of circNALCN (green) and miR-493-3p (red) in cells (magnification, 200×, scale bar, 50 µm). E RNA pulldown assay was executed in U87 and U251 cells, followed by qRT-PCR to detect the expression of miR-493-3p. F The proliferative abilities of U87 and U251 cells transfected with vector and miR-493-3p NC, oe-circNALCN and miR-493-3p mimics, oe-circNALCN and miR-493-3p NC, si-NC and miR-493-3p NC, si-circNALCN and miR-493-3p inhibitor, and si-circNALCN and miR-493-3p NC was detected by CCK-8. G, H The migration abilities of U87 and U251 cells transfected with vector and miR-493-3p NC, oe-circNALCN and miR-493-3p mimics, oe-circNALCN and miR-493-3p NC, si-NC and miR-493-3p NC, si-circNALCN and miR-493-3p inhibitor, and si-circNALCN and miR-493-3p NC was tested by wound healing test and Transwell assay. Scale bar: 100 µm. Data are presented as the mean ± SD; ns indicates no significance. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001. Vector: empty vector control; NC: negative control oe-circNALCN: circNALCN overexpression vector; si-NC: negative control siRNA; si-circNALCN: circNALCN siRNA