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Table 1 Primer sequence of genes for RT-qPCR

From: Overexpression of DDIT4 and TPTEP1 are associated with metastasis and advanced stages in colorectal cancer patients: a study utilizing bioinformatics prediction and experimental validation

Gene groups

Gene names

Primer Sequence (5´ → 3´)

Selected genes from bioinformatics analysis

DDIT4

F: CTTTGGGACCGCTTCTCGTC

R: GGTAAGCCGTGTCTTCCTCCG

SULF1

F: GGACGGATACAGCAGGAACG

R: CAGCACATGGGTGTAGTCACA

TPTEP1

F: AGCCGCAGACAAAAGACCTCGG

R: CCACCAAACAGGCTTCGTGTGA

miRNA-181d-5p

AACATTCATTGTTGTCGGTGGGT

miRNA-148b-3p

TCAGTGCATCACAGAACTTTGT

Stemness genes

OCT4

F: GTGGAGAGCAACTCCGATG

R: TGCAGAGCTTTGATGTCCTG

SOX2

F: AATGGGAGGGGTGCAAAAGAGG

R: GTGAGTGTGGATGGGATTGGTG

C-MYC

F: ACACATCAGCACAACTACG

R: CGCCTCTTGACATTCTCC

KLF4

F: CCTCGCCTTACACATGAAGAG

R: CATCGGGAAGACAGTGTGAAA

NANOG

F: AGCTACAAACAGGTGAAGAC

R: GGTGGTAGGAAGAGTAAAGG

EMT genes

TWIST1

F: TTCTCGGTCTGGAGGATGGAG

R: ACGCCCTGTTTCTTTGAATTTGG

SNAIL1

F: CCAGAGTTTACCTTCCAGCA

R: GATGAGCATTGGCAGCGA

VIM

F: TCTACGAGGAGGAGATGCGG

R: GGTCAAGACGTGCCAGAGAC

ZEB1

F: CTTCTCACACTCTGGGTCTTATTC

R: CGTTCTTCCGCTTCTCTCTTAC

ABC transporter genes

ABCB1

F: GTTCAGGTGGCTCTGGATAAG

R: AGCGATGACGTCAGCATTAC

ABCG2

F: TTCCACGATATGGATTTACGG

R: GTTTCCTGTTGCATTGAGTCC

ABCC1

F: CGCCTTCGCTGAGTTCCT

R: TGCGGTGCTGTTGTGGTG

Housekeeping genes

GAPDH

F: CATGAGAAGTATGACAACAGCCT

R: AGTCCTTCCACGATACCAAAGT

RNU6 (U6)

F: TCGCTTCGGCAGCACATATAC

R: GCGTGTCATCCTTGAGCAG