Fig. 2

Effects of CD146 on progression of OS cells in vitro. A Inhibitory role of ab75769 in proliferation of 143B cells at four-tier concentrations for 48 h (n = 6). B The proliferation of MG63-2 and 143B treated was analyzed by MTT assay (n = 6). Vector-transfected MG63 and 143B treated with IgG served as blank controls. C The migration of MG63-2 and143B was detected by using Transwell system (n ≥ 4). D The adhesion capability between OS and type I collagen was quantitatively measured for 30 min, 1 h or 3 h, respectively (n = 5). E The matrix colony formation was observed at MG63-2 and 143B (n = 3). Unless otherwise special explanation, in this study, the vector referred specifically to MG63 empty vector group; and IgG, ab75769 acted on 143B cells. Scale: × 100. *P < 0.05; **P < 0.01; ***P < 0.001