Fig. 2
Circ_0001721 knockdown impeded DXR resistance and tumor progression in DXR-resistant OS cells. A KHOS/DXR and MG63/DXR cells were introduced with si-NC, si-circ#1, si-circ#2 or si-circ#3, and the knockdown efficiency was examined by qRT-PCR. B After DXR treatment with gradient concentration, IC50 value was evaluated using CCK-8 assay. C, D CCK-8 assay was used to assess cell viability. E, F Transwell assay was utilized to evaluate cell migration and invasion. G Flow cytometry was used to monitor the apoptosis rate. H The levels of multidrug resistance-related proteins (MRP1, P-gp and LRP) were examined by western blot. I The activity of Wnt/β-catenin pathway was evaluated by detecting the protein levels of β-catenin, cyclin D1 and c-myc. J, K The protein levels of MRP1, P-gp, LRP, β-catenin, cyclin D1 and c-myc were measured in KHOS and MG63 cells transfected with si-NC or si-circ#2. *P < 0.05