Fig. 3

Circ_0001721 was a sponge of miR-758. A The putative binding sites of circ_0001721 and miR-758 were exhibited. B, C Luciferase activity was detected by dual-luciferase reporter assay in KHOS/DXR and MG63/DXR cells co-transfected with circ_0001721 WT or circ_0001721 MUT and miR-758 or miR-NC. D RIP assay was performed to confirm whether circ_0001721 bound to miR-758. E, F RNA pull-down assay was used to verify the relationship between circ_0001721 and miR-758. G Expression of miR-758 in Chemosensitive and Chemoresistant OS patients was detected by qRT-PCR. H The expression of miR-758 was measured using qRT-PCR in KHOS, KHOS/DXR, MG63 and MG63/DXR cells. I The expression level of miR-758 was detected in KHOS/DXR and MG63/DXR cells transfected with pcDNA or circ_0001721. *P < 0.05