Fig. 6
TCF4 alleviated the inhibition of miR-758 on DXR resistance in DXR-resistant OS cells. KHOS/DXR and MG63/DXR cells were transfected with miR-NC, miR-758, miR-758 + pcDNA or miR-758 + TCF4, respectively. A The protein expression of TCF4 was detected using western blot. B, C After treatment with different concentrations of DXR, IC50 was determined by CCK-8 assay. D, E CCK-8 analysis was used to assess cell viability. F, G Transwell assay was utilized to determine cell migration and invasion. (H) The cell apoptosis rate was monitored by flow cytometry. I and J The levels of multidrug resistance markers and Wnt/β-catenin pathway-related proteins were detected by western blot. *P < 0.05