Fig. 4

NEAT1 regulates the MEST/ATGL axis by regulating let-7 g. A The binding site of let-7 g and MEST predicted using starBase. B MEST expression in ovarian cancer samples in the TCGA database. C The binding of let-7 g to MEST verified by dual luciferase reporter gene assay. *p < 0.05 vs. the NC-mimic group. D Co-expression analysis of MEST and ATGL expression in ovarian cancer samples within the TCGA database. The x-axis represents the expression of MEST, and the y-axis represents the expression of ATGL. E The expression of let-7 g, MEST and ATGL in HO8910 and SKOV3 cells after silencing of let-7 g determined by RT-qPCR. F The protein expression of MEST and ATGL in HO8910 and SKOV3 cells after silencing of let-7 g determined by western blot analysis. G The expression levels of MEST and ATGL in HO8910 and SKOV3 cells after silencing NEAT1, as determined by RT-qPCR. H The protein expression of MEST and ATGL in HO8910 and SKOV3 cells after silencing NEAT1 determined by western blot analysis. I MEST expression in ovarian cancer tissues and adjacent normal tissues determined by RT-qPCR, n = 68. J, ATGL expression in ovarian cancer tissues and adjacent normal tissues determined by RT-qPCR, n = 68. K Positive expression of MEST and ATGL proteins in ovarian cancer tissues and adjacent normal tissues determined by immunohistochemistry. L Pearson correlation analysis of let-7 g expression with MEST expression in clinical samples, n = 68. In figure D, E *p < 0.05 vs. the NC-mimic group. #p < 0.05 vs. the NC-inhibitor group. In figure F, G *p < 0.05 vs. the oe-NC group. #p < 0.05 vs. the si-NC group. In figure H, I *p < 0.05 vs. adjacent normal tissues. The measurement data were expressed as mean ± standard deviation. Comparisons between two groups were conducted by t-test, while comparisons among multiple groups were assessed by one-way ANOVA followed by Tukey's post hoc test. All cell experiments were performed 3 times independently