Fig. 2

LINC00115 promotes the maintenance of OCSC stemness and inhibits its apoptosis. Two sh-LINC00115 vectors (sh1 and sh2) and their negative control (Ctrl) were used to transfect CD133⁺ OCSCs cells. A The expression heat map of 50 DEGs/lncRNAs on GSE66957, in which Y-axis represents DEGs/lncRNAs. The upper right histogram is the color order, and each rectangle corresponds to a sample expression value; B Relative expression of LINC00115 in A2780 cells and CD133⁺ OCSCs detected by RT-qPCR, compared with the A2780 cells, *p < 0.05; C The sphere-forming rate of CD133⁺ OCSCs was observed under a microscope, VS the Ctrl group, ***p < 0.001; D, E. Relative expression of LINC00115, CD133, CD44, NANOG and POU5F1 in CD133⁺ OCSCs was detected using RT-qPCR and Western blot analysis, vs the Ctrl group, *p < 0.05; F Cell apoptosis was measured using flow cytometry; G. Relative Caspase-3 activity in cells of each group, VS the Ctrl group, *p < 0.05; H. Percentage of DNA damage in cell supernatant of each group, VS the Ctrl group, *p < 0.05. Data in panel B were analyzed using the unpaired t test, and the data in panels C-H were analyzed using one-way ANOVA and Tukey’s multiple comparison test. The experiment was performed in triplicate and data were expressed as means ± standard deviation