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Fig. 3 | Cancer Cell International

Fig. 3

From: LINC00115 promotes stemness and inhibits apoptosis of ovarian cancer stem cells by upregulating SOX9 and inhibiting the Wnt/β-catenin pathway through competitively binding to microRNA-30a

Fig. 3

LINC00115 could bind to miR-30a. A Comparing the predicted results of LINC00115-targeted miRs from the miRcode and RNAInter databases, there were five intersecting miRs; B The expression heat map of 10 differentially expressed miRs on GSE48485, in which Y-axis represents miRs. The upper right histogram is the color order, and each rectangle corresponds to a sample expression value; C The binding sites between LINC00115 and miR-30a were analyzed by RNAInter database; D-F. LNC00115 could bind to miR-30a verified by dual luciferase reporter gene assay (D, vs the mimic-NC group, **p < 0.01), RNA pull-down assay (E, vs the Bio-miR-30a-mut group, **p < 0.01) and RIP assay (F, vs the IgG group, ***p < 0.001). Data in panel D were analyzed using the unpaired t test, and data in panels E–F were analyzed using one-way ANOVA and Tukey’s multiple comparison test. Repetitions = 3. The experiment was performed in triplicate and data were expressed as means ± standard deviation

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Cancer Cell International

ISSN: 1475-2867

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