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Fig. 4 | Cancer Cell International

Fig. 4

From: LINC00115 promotes stemness and inhibits apoptosis of ovarian cancer stem cells by upregulating SOX9 and inhibiting the Wnt/β-catenin pathway through competitively binding to microRNA-30a

Fig. 4

miR-30a could target SOX9. A TargetScan, DIANA, miRSearch and miRDB databases predicted the target genes of miR-30a, and the predicted results were compared with the DEGs on GSE26712 and GSE4122; B, C Differential expression of SOX9 and NT5E on GSE26712 and GSE4122; D TargetScan software predicted the potential binding sites of miR-30a to SOX9; E Luciferase activity detected the targeting relationship between miR-30a and SOX9; vs the mimic-NC cells, **p < 0.01; F-G: Relative SOX9 expression in CD133+ OCSCs detected by RT-qPCR and Western blot analysis, vs mimic-NC cells, *p < 0.05; H. Relative expression of SOX9 and miR-30a in CD133+ OCSCs after silencing LINC00115 detected by RT-qPCR. Compared with the Ctrl group, *p < 0.05, **p < 0.01. Data in panels E–G were analyzed using the unpaired t test, while data in panel H were analyzed using one-way ANOVA and Tukey’s multiple comparison test. The experiment was performed in triplicate and data were expressed as means ± standard deviation

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