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Fig. 7 | Cancer Cell International

Fig. 7

From: LINC00115 promotes stemness and inhibits apoptosis of ovarian cancer stem cells by upregulating SOX9 and inhibiting the Wnt/β-catenin pathway through competitively binding to microRNA-30a

Fig. 7

Wnt/β-catenin pathway participates in the maintenance of OCSCs. OCSCs stably transfected with sh1 were delivered miR-30a inhibitor or SOX9-vector, or 5 mM Wnt/β-catenin specific activator SKL2001. A, B. Western blot analysis detected the expression of Wnt/β-catenin pathway; C. Microscopic observation of spheres (100 ×) and sphere-forming rate of OCSCs measured by sphere-forming assay; D Relative levels of CD133, SOX9, CD44, NANOG, and POU5F1 in CD133+ OCSCs were measured using Western blot analysis; E Cell apoptosis was measured by flow cytometry; F Relative Caspase-3 activity; G. Relative percentage of DNA damage in cell supernatant of each group. VS the sh1 + PBS group, * p < 0.05. Data in panels A and B were analyzed using one-way ANOVA and Tukey’s multiple comparison test, while data in panels C and F were analyzed using the unpaired t test. The experiment was performed in triplicate and data were expressed as means ± standard deviation

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Cancer Cell International

ISSN: 1475-2867

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